(2) FC1， (4) AX IFC Unit3的Fluidigm BioMark HD Real-Time PCR

下面是当前型号Biomark X和Biomark HD/Juno组合的比较.
 

• 配置4个IFC控制器和4个fc1时, the Biomark HD can perform up to 147,456 Genotypes per day. (16 runs in an 8hr period)
• 最新的Biomark X每天8小时运行约2次，每天限制为18432个基因型. (2.2 runs in an 8 hour period)
• 为Covid测试配置并发并行运行时, the Biomark HD system can test 6,000 samples and controls per day. (使用Advanta Dx SARS-CoV-2 RT-PCR检测，Juno控制器RX)
• Biomark HD匹配Biomark X每次运行的数据点和反应
• Biomark X can run the 96.基因表达和基因分型分别为96和48.48 Dynamic array for Digital PCR
• Biomark HD可以运行与Biomark X + 192相同的ifc.24 Dynamic Array, Flex Six, 12.765 Digital Array, qdPCR 37K IFC, and the 48.48可用于基因表达和基因分型增加灵活性.
• BioMark HD在荧光发射范围上与BioMark X相似.
• Biomark X和Biomark HD在基于peltier的热控制和性能上相似

整个系统是在2020年购买并安装的，最初的成本约为30万美元.00

Predecessor to:
Current model.

Condition:
This Fluidigm BioMark HD Real-Time PCR system is in good working order.

Please see the exact video and photos below.

Testing we performed:
The Fluidigm BioMark HD Real-Time PCR 系统通过了所有测试，功能齐全:
HD Unit:
Ran ICP program onboard testing: PASSED
IFC chips identified: PASSED
相机聚焦荧光和环境光:通过
Thermal cycler temps verified: PASSED
分析程序加载演示文件并分析:PASSED

Biomark AX IFC controllers:
Tested vacuum seal integrity: PASSED
Tested cleaning plate cycle: PASSED
Tested all touchscreen controls: PASSED
测试控制器识别芯片和拒绝不兼容芯片:通过
以Admin登录并验证保存的协议:PASSED

Fluidigm FC1 Cyclers:
Tested all touchscreen controls: PASSED
Ran 48x48 Standard v1 protocol: PASSED

Fluidigm Juno Library Preparation System:
Tested all touchscreen controls: PASSED
Tested drawer function: PASSED
Ran IFC Control-Prime: PASSED

System consists of:
Fluidigm生物标记HD PCR系统(带显示器、键盘、鼠标)
Juno Library Preparation System
带有Juno装载夹具的MX和RX接口板
AX IFC Controllers (Qty 4)
FC1 Cyclers (Qty 2)
Communication and power cables.

Configuration and Setup:
Run Time: 96.96 IFC (9216 reactions) in under 3 hours
温度范围:基于珀尔蒂埃，4-99摄氏度，斜坡速率高达5.5C/sec
Detection: CCD camera; 9M pixel, 12 x 12um pixel size
荧光发射:525-25nm、570-30nm、645-75nm、700-30nm
荧光激发:485-20nm、530-20nm、580-25nm、632-27nm

Biomark HD PCR System:
Dimensions (w x d x h): 75 x 75 x 100cm (29.5" x 29.5" x 39.4")
Weight: 140kg (308lbs)
Power requirements: 100-230 VAC, 50/60 Hz, 300 W

Fluidigm Juno Library Preparation System:
Thermal control: Peltier-based, ranging from 4C to 99C ( Maximum heating rate: >4C/sec, Maximum cooling rate: >3C/sec)
Dimensions (w x d x h): 43.9 x 69.8 x 50cm (17.3"x27.5"x19.7")
Weight: 52kg (115lbs)

Fluidigm AX IFC Controller:
Dimensions (w x d x h): 24.1 x 48.3 x 33cm (9.5"x19"x13")
Weight: 27kg (59.4lbs)

Fluidigm FC1 Cycler:
外形尺寸(宽x深x高):23 x 48 x 20cm(9”x19”x8”)
Weight: 11kg (25lbs)

Video Description:
Video 1 gives an overview of the Fluidigm BioMark HD Real-Time PCR system and Juno Library Preparation System.
它演示了Biomark HD装载IFC并开始运行，而Juno启动IFC板.

视频2给出了IFC控制器和FC1循环器的概述, 演示抽屉功能和状态屏幕.

Documentation:
FC1 Setup Guide: http://drive.google.com/file/d/1BOJBVnL0lxkdEg4u2gO2EX6ijUuSaCxY/view?usp=sharing

HD System Brochure: http://drive.google.com/file/d/15O-W_iGBBmbYPWo_BLBslcBtdX-AXquP/view?usp=sharing

FC1 Manual: http://drive.google.com/file/d/1de4G36XPdl3GnQffRG6A9DEv_qlWkpiJ/view?usp=sharing

Juno Manual: http://drive.google.com/file/d/1yH9s3xNZ7JqlvRr9QMK3dl1f9sxWZ5Qi/view?usp=sharing

SNP Genotyping Manual: http://drive.google.com/file/d/1544OcqHClw7nKqufsDC630MCoW9HuZhZ/view?usp=sharing

Data Collection Manual: http://drive.google.com/file/d/1ZwRAun07mFjZlpXgfcXYhybmGCM9jmTL/view?usp=sharing

AX Controllers Manual: http://drive.google.com/file/d/1TDt8ri2zXxuoUC6ylBY3zEFgr_s6wDVA/view?usp=sharing

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Video #1

Video #2